Researchers have developed a groundbreaking Roxithromycin detection biosensor that utilizes a target-induced DNA nanodevice. This system combines a regenerable Surface-Enhanced Raman Scattering (SERS) substrate with an advanced signal amplification strategy. Consequently, the sensor addresses common limitations such as substrate waste and poor detection stability. Additionally, the integration of these technologies ensures an ultrasensitive platform for clinical and pharmaceutical applications.

The core of this innovation involves the HOF@Au substrate, which researchers engineered by reducing gold nanoparticles on alkali-resistant HOF-102. Furthermore, the team designed a DNA triplex structure that responds directly to pH levels. When the target antibiotic is present, the specific aptamer activates a DNAzyme. This interaction triggers a series of cleavage and rolling-assembly cycles. Therefore, this cascade system amplifies the Raman signal significantly. This mechanism allows for the precise quantification of trace drug levels in various samples.

Advancing the Roxithromycin Detection Biosensor Technology

The proposed strategy demonstrates a remarkable limit of detection at 3.97 × 10^-14 mol/L. Notably, the system is fully regenerable due to the pH-responsive nature of the DNA structure. By modulating pH, the triplex structure folds or unfolds to allow for the efficient reuse of the SERS substrate. This capability simplifies testing procedures while significantly reducing overall laboratory costs. As a result, this multifunctional platform offers a sustainable model for the future of antibiotic monitoring and biosensing.

Frequently Asked Questions

What is the primary benefit of the HOF@Au substrate?

The HOF@Au substrate provides a uniform and high-enhancement surface for Raman signals. It is alkali-resistant and regenerable, which makes the detection process more stable and cost-effective than traditional single-use substrates.

How does the DNA nanodevice improve sensitivity?

The nanodevice uses a target-triggered cycling mechanism involving identifying, cleaving, and rolling-assembly steps. This cascade system amplifies the SERS signal, enabling the sensor to detect trace concentrations of Roxithromycin as low as 3.97 × 10^-14 mol/L.

Disclaimer: This content is for informational and educational purposes only. It is not intended as a substitute for professional medical advice, diagnosis, or treatment. Refer to the latest local and national guidelines for clinical practice.

References

1. Zhang R et al. Target-Induced DNA Nanodevice as Efficient Signal Amplification Strategy Coupled with a Regenerable SERS Substrate HOF@Au for Ultrasensitive Detection of Roxithromycin. Anal Chem. 2026 Mar 24. doi: 10.1021/acs.analchem.6c00885. PMID: 41873606.


2. Sultana S, et al. Recent advances in SERS-based biosensors for antibiotic detection in clinical and environmental samples. Sensors and Actuators B: Chemical. 2023;374:132791.


3. Jiang L, et al. Preparation and characterization of DNA aptamers against roxithromycin. Anal Chim Acta. 2021;1164:338509.