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The system is Rev-dependent. It specifically responds to HIV Tat and Rev proteins rather than being triggered by mitogens. This mechanism minimizes background noise usually seen with the LTR promoter.
Yes. The cell lines express CD4, CXCR4, and various levels of CCR5. Consequently, they are responsive to both X4 and R5-tropic viruses, including complex clade C swarms.
Disclaimer: This content is for informational and educational purposes only. It does not constitute medical advice or a substitute for professional healthcare. Always consult a qualified medical professional for diagnosis and treatment. Refer to the latest local and national guidelines for clinical practice.
References
Spear M et al. Versatile HIV Rev-dependent reporter cell system for stringent and sensitive quantification of viral reservoirs, neutralizing antibodies, and restriction factors. Retrovirology. 2026 Feb 11. doi: 10.1186/s12977-026-00675-8. PMID: 41673644.
Montefiori DC. Measuring HIV neutralization in a luciferase reporter gene assay. Methods Mol Biol. 2009;485:395-405.
Pitisuttithum P et al. HIV-1 Clade C Research and Vaccine Development. Front Immunol. 2021;12:656403.

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