.webp&w=384&q=75){kind=logo}
Loading, please wait...
Loading, please wait...
The manufacturing of modern biopharmaceuticals is evolving rapidly. Monoclonal antibody purification has emerged as a cornerstone in producing safe and effective treatments for cancer and autoimmune diseases. Multimodal chromatography (MMC) provides a powerful alternative to traditional methods. It integrates multiple interactions like hydrogen bonding and hydrophobic forces. Consequently, this technology significantly improves the selectivity of the purification process.
Recent studies emphasize the importance of resin selection in bioprocessing. This review compares all commercially available MMC resins. It analyzes performance metrics such as binding capacity and recovery rates. Furthermore, it evaluates the clearance of host cell proteins and DNA. These factors are crucial for maintaining the safety profile of mAb-based products. Therefore, manufacturers are adopting these advanced techniques to streamline production.
In addition to empirical data, the research incorporates molecular-level insights. Techniques such as NMR and molecular docking reveal how antibodies interact with chromatography ligands. This mechanistic understanding allows for a more rational selection of resins. Moreover, high-throughput screening and in silico modeling are now essential enablers. They help predict partition coefficients and optimize mobile phase modifiers.
As a result, these advances support the development of next-generation biopharmaceutical platforms. They ensure that complex molecules like bispecific antibodies and drug conjugates meet strict regulatory standards. Ultimately, improved efficiency in production may lead to better access to affordable biologics in India.
MMC offers higher selectivity compared to traditional ion-exchange or hydrophobic interaction chromatography. It combines multiple binding mechanisms, which allows for better removal of impurities like protein aggregates and host cell contaminants.
By achieving superior clearance of host cell proteins and DNA, MMC reduces the risk of immunogenic reactions in patients. This ensures that the final antibody product is of the highest therapeutic quality and meets rigorous safety standards.
Disclaimer: This content is for informational and educational purposes only. It does not constitute medical advice or professional biopharmaceutical consulting. Refer to the latest local and national guidelines for clinical practice.
References
1. Javidanbardan A et al. Multimodal Chromatography in the Downstream Processing of mAb-Based Products: Mechanisms, Strategies, and Applications. Biotechnol Bioeng. 2026 Feb 25. doi: 10.1002/bit.70171. PMID: 41738359.
2. Shukla AA, et al. Downstream processing of monoclonal antibodies--application of platform approaches. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Mar 15;848(1):28-39.
3. Halan V, et al. Multimodal Chromatography for Purification of Biotherapeutics - A Review. Curr Protein Pept Sci. 2019;20(1):4-13.
Read summarized clinical updates, watch expert medical content, and earn CME certifications right from your smartphone.
Open your smartphone camera to scan and install.
A comprehensive review of multimodal chromatography in mAb production, focusing on mechanisms, resin selection, and its impact on biopharmaceutical purity....
4 months ago
A recent cross-sectional study of 15.2 million adults reveals a goiter prevalence of 1.3-2.5%. This detailed analysis explores why women and older adults are at higher risk, the dominance of non-toxic multinodular subtypes, and the clinical implications for primary care and endocrinology.
Today
A deep dive into the National Family Health Survey (NFHS-6) data showing a sharp rise in surgical deliveries in Karnataka, the public-private healthcare divide, clinical risks of elective surgeries, and the urgent need for standardized medical guidelines to safeguard maternal and newborn health.
Today
A breakthrough study has utilized computational fluid dynamics to develop an optimized multi-stage drainage cannula for VA ECMO. By refining side-hole placement and angles, the new design significantly reduces stagnant blood volumes and increases flow efficiency, potentially lowering the risk of thrombosis.
Today
Perilunate dislocations are challenging orthopedic emergencies. This article explores a novel 4-anchor suture tape internal bracing technique that eliminates the need for K-wire retention, facilitating earlier recovery and excellent long-term functional outcomes as demonstrated in a recent clinical case.
Today
An international panel of experts has officially recognized fatty pancreas disorder as a distinct disease in the Melbourne Consensus. Learn about its diagnostic criteria using MRI, its pathophysiological association with type 2 diabetes and cancer, and evidence-based lifestyle and pharmacological interventions.
Today
